Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface.

Taiqoui Pelá, Vinícius; Buzalaf, Marília Afonso Rabelo; Niemeyer, Samira; Baumann, Tommy; Henrique-Silva, Flávio; Toyama, Danyelle; Crusca, Edson; Marchetto, Reinaldo; Lussi, Adrian; Saads Carvalho, Thiago (2021). Acquired pellicle engineering with proteins/peptides: Mechanism of action on native human enamel surface. Journal of dentistry, 107, p. 103612. Elsevier Science 10.1016/j.jdent.2021.103612

Text 1-s2.0-S0300571221000336-main.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (4MB)

OBJECTIVE

This study investigated the mechanism of action of different proteins/peptides (separately or in combination), focusing on how they act directly on the native enamel surface and on modifying the salivary pellicle.

METHODS

A total of 170 native human enamel specimens were prepared and submitted to different treatments (2 h; 37 °C): with deionized water, CaneCPI-5, Hemoglobin, Statherin, or a combination of all three proteins/peptides. The groups were subdivided into treatment acting on the enamel surface (NoP - absence of salivary pellicle), and treatment modifying the salivary pellicle (P). Treatment was made (2 h; 37 °C) in all specimens, and later, for P, the specimens were incubated in human saliva (2 h; 37 °C). In both cases, the specimens were immersed in 1% citric acid (pH 3.6; 2 min; 25 °C). Calcium released from enamel (CaR) and its relative surface reflection intensity (%SRI) was measured after 5 cycles. Between-group differences were verified with two-way ANOVA, with "presence of pellicle" and "treatment" as factors (α = 0.05).

RESULTS

The presence of pellicle provided better protection regarding %SRI (p < 0.01), but not regarding CaR (p = 0.201). In relation to treatment, when compared to the control group, all proteins/peptides provided significantly better protection (p < 0.01 for %SRI and Car). The combination of all three proteins/peptides demonstrated the best protective effect (p < 0.01 for %SRI).

CONCLUSION

Depending on the protein or peptide, its erosion-inhibiting effect derives from their interaction with the enamel surface or from modifying the pellicle, so a combination of proteins and peptides provides the best protection.

CLINICAL SIGNIFICANCE

The present study opens a new direction for a possible treatment with a combination of proteins for native human enamel, which can act directly on the enamel surface as well on the modification of the salivary pellicle, for the prevention of dental erosion.

Interest & Impact

Downloads

1 since deposited on 05 Jan 2022
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item