Russell, Gabriel A; Peng, Garrie; Faubert, Cynthia; Verdu, Elena F; Hapfelmeier, Siegfried; King, Irah L (2021). A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies. STAR protocols, 2(4), p. 100946. Elsevier 10.1016/j.xpro.2021.100946
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The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research |
UniBE Contributor: |
Hapfelmeier, Siegfried Hektor |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2666-1667 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Siegfried Hektor Hapfelmeier-Balmer |
Date Deposited: |
24 Jan 2022 13:36 |
Last Modified: |
09 Jul 2024 21:27 |
Publisher DOI: |
10.1016/j.xpro.2021.100946 |
PubMed ID: |
34825215 |
Uncontrolled Keywords: |
Cell culture Health Sciences Immunology Microbiology |
BORIS DOI: |
10.48350/163849 |
URI: |
https://boris.unibe.ch/id/eprint/163849 |
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