A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies.

Russell, Gabriel A; Peng, Garrie; Faubert, Cynthia; Verdu, Elena F; Hapfelmeier, Siegfried; King, Irah L (2021). A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies. STAR protocols, 2(4), p. 100946. Elsevier 10.1016/j.xpro.2021.100946

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The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research

UniBE Contributor:

Hapfelmeier, Siegfried Hektor

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2666-1667

Publisher:

Elsevier

Language:

English

Submitter:

Siegfried Hektor Hapfelmeier-Balmer

Date Deposited:

24 Jan 2022 13:36

Last Modified:

05 Dec 2022 16:01

Publisher DOI:

10.1016/j.xpro.2021.100946

PubMed ID:

34825215

Uncontrolled Keywords:

Cell culture Health Sciences Immunology Microbiology

BORIS DOI:

10.48350/163849

URI:

https://boris.unibe.ch/id/eprint/163849

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