Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology.

Shumilov, Evgenii; Mazzeo, Paolo; Zinkernagel, Martin S.; Legros, Myriam; Porret, Naomi; Romagna, Lorenz; Haase, Detlef; Lenz, Georg; Novak, Urban; Banz, Yara; Pabst, Thomas; Bacher, Vera (2022). Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology. Current oncology, 29(2), pp. 766-776. MDPI 10.3390/curroncol29020065

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BACKGROUND

Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the workup algorithm of IOL and compared its sensitivity with the results obtained from routine cytopathology and molecular genetics; Methods: Seven patients undergoing vitrectomy due to suspected IOL were investigated by FC and parallel cytopathology and, if available, digital droplet PCR (ddPCR) for MYD88 L265P; Results: Four out of seven patients were finally diagnosed with IOL. Among the IOL patients, cytopathology confirmed the presence of lymphoma cells in only two cases. In contrast, FC was positive for IOL in all four cases, and FC additionally confirmed the lack of IOL in the remaining patients. In IOL patients diagnosed by FC and with available ddPCR, the diagnosis of IOL was confirmed by the presence of the MYD88 L265P mutation in all three patients; Conclusions: The combination with FC was superior to cytopathology alone in the diagnostic work-up of IOL, and it showed an excellent correlation with ddPCR results. A comprehensive diagnostic panel consisting of cytopathology, FC and molecular genetics should be considered for the work-up of suspected IOL.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Haematology and Central Haematological Laboratory
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Medical Oncology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Zinkernagel, Martin Sebastian; Legros, Myriam; Porret, Naomi; Romagna, Lorenz; Novak, Urban; Banz Wälti, Yara Sarah; Pabst, Thomas Niklaus and Bacher, Vera Ulrike

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1718-7729

Publisher:

MDPI

Language:

English

Submitter:

Pubmed Import

Date Deposited:

25 Feb 2022 10:19

Last Modified:

25 Feb 2022 10:24

Publisher DOI:

10.3390/curroncol29020065

PubMed ID:

35200564

Uncontrolled Keywords:

MYD88 cytopathology flow cytometry intraocular lymphoma molecular genetics vitreous body

BORIS DOI:

10.48350/166028

URI:

https://boris.unibe.ch/id/eprint/166028

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