Glucose Transporter 9 (GLUT9) Plays an Important Role in the Placental Uric Acid Transport System.

Lüscher, Benjamin P.; Albrecht, Christiane; Stieger, Bruno; Surbek, Daniel V.; Baumann, Marc U. (2022). Glucose Transporter 9 (GLUT9) Plays an Important Role in the Placental Uric Acid Transport System. Cells, 11(4) MDPI 10.3390/cells11040633

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BACKGROUND

Hyperuricemia is a common laboratory finding in pregnant women compromised by preeclampsia. A growing body of evidence suggests that uric acid is involved in the pathogenesis of preeclampsia. Glucose transporter 9 (GLUT9) is a high-capacity uric acid transporter. The aim of this study was to investigate the placental uric acid transport system, and to identify the (sub-) cellular localization of GLUT9.

METHODS

Specific antibodies against GLUT9a and GLUT9b isoforms were raised, and human villous (placental) tissue was immunohistochemically stained. A systemic GLUT9 knockout (G9KO) mouse model was used to assess the placental uric acid transport capacity by measurements of uric acid serum levels in the fetal and maternal circulation.

RESULTS

GLUT9a and GLUT9b co-localized with the villous (apical) membrane, but not with the basal membrane, of the syncytiotrophoblast. Fetal and maternal uric acid serum levels were closely correlated. G9KO fetuses showed substantially higher uric acid serum concentrations than their mothers.

CONCLUSIONS

These findings demonstrate that the placenta efficiently maintains uric acid homeostasis, and that GLUT9 plays a key role in the placental uric acid transport system, at least in this murine model. Further studies investigating the role of the placental uric acid transport system in preeclampsia are eagerly needed.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Lüscher, Benjamin P.; Albrecht, Christiane; Surbek, Daniel and Baumann, Marc

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

2073-4409

Publisher:

MDPI

Language:

English

Submitter:

Pubmed Import

Date Deposited:

01 Mar 2022 14:24

Last Modified:

01 Mar 2022 14:33

Publisher DOI:

10.3390/cells11040633

PubMed ID:

35203284

Uncontrolled Keywords:

GLUT9 glucose transporter 9 preeclampsia uric acid

BORIS DOI:

10.48350/166114

URI:

https://boris.unibe.ch/id/eprint/166114

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