Rutland, Catrin S; Cockcroft, James M; Lothion-Roy, Jennifer; Harris, Anna E; Jeyapalan, Jennie N; Simpson, Siobhan; Alibhai, Aziza; Bailey, Clara; Ballard-Reisch, Alyssa C; Rizvanov, Albert A; Dunning, Mark D; De Brot, Simone; Mongan, Nigel P (2021). Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma. Frontiers in veterinary science, 8, p. 704598. Frontiers Media 10.3389/fvets.2021.704598
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Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous OSA in dogs provides a good model for the disease seen in people and also remains an important veterinary clinical challenge. We recently used RNA sequencing and qRT-PCR to provide a detailed molecular characterization of OSA relative to non-malignant bone in dogs. We identified differential mRNA expression of the solute carrier family 2 member 1 (SLC2A1/GLUT1), matrix metallopeptidase 3 (MMP3) and nuclear factor erythroid 2-related factor 2 (NFE2L2/NRF2) genes in canine OSA tissue in comparison to paired non-tumor tissue. Our present work characterizes protein expression of GLUT1, MMP3 and NRF2 using immunohistochemistry. As these proteins affect key processes such as Wnt activation, heme biosynthesis, glucose transport, understanding their expression and the enriched pathways and gene ontologies enables us to further understand the potential molecular pathways and mechanisms involved in OSA. This study further supports spontaneous OSA in dogs as a model system to inform the development of new methods to diagnose and treat OSA in both dogs and people.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology |
UniBE Contributor: |
De Brot, Simone Danielle |
Subjects: |
500 Science 500 Science > 570 Life sciences; biology 500 Science > 590 Animals (Zoology) 600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
2297-1769 |
Publisher: |
Frontiers Media |
Language: |
English |
Submitter: |
Katharina Gerber-Paizs |
Date Deposited: |
08 Mar 2022 18:54 |
Last Modified: |
05 Dec 2022 16:11 |
Publisher DOI: |
10.3389/fvets.2021.704598 |
PubMed ID: |
34414229 |
Uncontrolled Keywords: |
cancer identification canine matrix metallopeptidase 3 nuclear factor erythroid 2-related factor 2 osteosarcoma pathology solute carrier family 2 member 1 |
BORIS DOI: |
10.48350/166249 |
URI: |
https://boris.unibe.ch/id/eprint/166249 |
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