Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek.

Neradilová, Silvie; Schauer, Alexandria Marie; Hayward, Jessica J; Brunner, Magdalena Anna Theresa; Bohutínská, Magdalena; Jagannathan, Vidhya; Connell, Laurie B; Boyko, Adam R; Welle, Monika M; Černá Bolfíková, Barbora (2022). Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek. Genes, 13(4) MDPI, Molecular Diversity Preservation International 10.3390/genes13040650

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Non-inflammatory alopecia is a frequent skin problem in dogs, causing damaged coat integrity and compromised appearance of affected individuals. In this study, we examined the Cesky Fousek breed, which displays atypical recurrent flank alopecia (aRFA) at a high frequency. This type of alopecia can be quite severe and is characterized by seasonal episodes of well demarcated alopecic areas without hyperpigmentation. The genetic component responsible for aRFA remains unknown. Thus, here we aimed to identify variants involved in aRFA using a combination of histological, genomic, and transcriptomic data. We showed that aRFA is histologically similar to recurrent flank alopecia, characterized by a lack of anagen hair follicles and the presence of severely shortened telogen or kenogen hair follicles. We performed a genome-wide association study (GWAS) using 216 dogs phenotyped for aRFA and identified associations on chromosomes 19, 8, 30, 36, and 21, highlighting 144 candidate genes, which suggests a polygenic basis for aRFA. By comparing the skin cell transcription pattern of six aRFA and five control dogs, we identified 236 strongly differentially expressed genes (DEGs). We showed that the GWAS genes associated with aRFA are often predicted to interact with DEGs, suggesting their joint contribution to the development of the disease. Together, these genes affect four major metabolic pathways connected to aRFA: collagen formation, muscle structure/contraction, lipid metabolism, and the immune system.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Research Foci > DermFocus

UniBE Contributor:

Schauer, Alexandria Marie; Brunner, Magdalena Anna Theresa; Jagannathan, Vidya and Welle, Monika Maria

Subjects:

600 Technology > 630 Agriculture
500 Science > 590 Animals (Zoology)

ISSN:

2073-4425

Publisher:

MDPI, Molecular Diversity Preservation International

Language:

English

Submitter:

Pubmed Import

Date Deposited:

26 Apr 2022 14:45

Last Modified:

26 Apr 2022 14:54

Publisher DOI:

10.3390/genes13040650

PubMed ID:

35456456

Uncontrolled Keywords:

Cesky Fousek GWAS RNA-seq atypical recurrent flank alopecia canine alopecia differential gene expression dog skin biopsies

BORIS DOI:

10.48350/169488

URI:

https://boris.unibe.ch/id/eprint/169488

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