Fahmi, Amal; Brügger, Melanie; Zumkehr, Beatrice; Oliveira Esteves, Blandina I; Baud, David; Alves, Marco P (2022). Generation of precision-cut slice cultures of human placenta. STAR protocols, 3(2), p. 101291. Elsevier 10.1016/j.xpro.2022.101291
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We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).
Item Type: |
Journal Article (Further Contribution) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Fahmi, Amal, Brügger, Melanie, Oliveira Esteves Criblez, Blandina Isabel, Alves, Marco |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2666-1667 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
26 Apr 2022 09:36 |
Last Modified: |
05 Dec 2022 16:19 |
Publisher DOI: |
10.1016/j.xpro.2022.101291 |
PubMed ID: |
35463475 |
Uncontrolled Keywords: |
Cell Biology Cell culture Developmental biology Health Sciences |
BORIS DOI: |
10.48350/169520 |
URI: |
https://boris.unibe.ch/id/eprint/169520 |
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