Microbial uptake in oral mucosa-draining lymph nodes leads to rapid release of cytotoxic CD8+ T cells lacking a gut-homing phenotype.

Barreto de Albuquerque, Juliana; Altenburger, Lukas M; Abe, Jun; von Werdt, Diego; Wissmann, Stefanie; Martínez Magdaleno, Jose; Francisco, David; van Geest, Geert; Ficht, Xenia; Iannacone, Matteo; Bruggmann, Remy; Mueller, Christoph; Stein, Jens V (2022). Microbial uptake in oral mucosa-draining lymph nodes leads to rapid release of cytotoxic CD8+ T cells lacking a gut-homing phenotype. Science immunology, 7(72), eabf1861. American Association for the Advancement of Science 10.1126/sciimmunol.abf1861

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The gastrointestinal (GI) tract constitutes an essential barrier against ingested microbes, including potential pathogens. Although immune reactions are well studied in the lower GI tract, it remains unclear how adaptive immune responses are initiated during microbial challenge of the oral mucosa (OM), the primary site of microbial encounter in the upper GI tract. Here, we identify mandibular lymph nodes (mandLNs) as sentinel lymphoid organs that intercept ingested Listeria monocytogenes (Lm). Oral Lm uptake led to local activation and release of antigen-specific CD8+ T cells that constituted most of the early circulating effector T cell (TEFF) pool. MandLN-primed TEFF disseminated to lymphoid organs, lung, and OM and contributed substantially to rapid elimination of target cells. In contrast to CD8+ TEFF generated in mesenteric LN (MLN) during intragastric infection, mandLN-primed TEFF lacked a gut-seeking phenotype, which correlated with low expression of enzymes required for gut-homing imprinting by mandLN stromal and dendritic cells. Accordingly, mandLN-primed TEFF decreased Lm burden in spleen but not MLN after intestinal infection. Our findings extend the concept of regional specialization of immune responses along the length of the GI tract, with CD8+ TEFF generated in the upper GI tract displaying homing profiles that differ from those imprinted by lymphoid tissue of the lower GI tract.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Immunopathology

UniBE Contributor:

Barreto de Albuquerque, Juliana; von Werdt, Diego; Ferreira Francisco, David Miguel; van Geest, Gerrit Adriaan; Bruggmann, Rémy and Müller, Christoph

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2470-9468

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Pubmed Import

Date Deposited:

22 Jun 2022 08:24

Last Modified:

22 Jun 2022 08:33

Publisher DOI:

10.1126/sciimmunol.abf1861

PubMed ID:

35714202

BORIS DOI:

10.48350/170765

URI:

https://boris.unibe.ch/id/eprint/170765

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