LITOS: a versatile LED illumination tool for optogenetic stimulation.

Höhener, Thomas Christoph; Landolt, Alex Erich; Dessauges, Coralie; Hinderling, Lucien; Gagliardi, Paolo Armando; Pertz, Olivier (2022). LITOS: a versatile LED illumination tool for optogenetic stimulation. Scientific reports, 12(1), p. 13139. Springer Nature 10.1038/s41598-022-17312-x

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Optogenetics has become a key tool to manipulate biological processes with high spatio-temporal resolution. Recently, a number of commercial and open-source multi-well illumination devices have been developed to provide throughput in optogenetics experiments. However, available commercial devices remain expensive and lack flexibility, while open-source solutions require programming knowledge and/or include complex assembly processes. We present a LED Illumination Tool for Optogenetic Stimulation (LITOS) based on an assembled printed circuit board controlling a commercially available 32 × 64 LED matrix as illumination source. LITOS can be quickly assembled without any soldering, and includes an easy-to-use interface, accessible via a website hosted on the device itself. Complex light stimulation patterns can easily be programmed without coding expertise. LITOS can be used with different formats of multi-well plates, petri dishes, and flasks. We validated LITOS by measuring the activity of the MAPK/ERK signaling pathway in response to different dynamic light stimulation regimes using FGFR1 and Raf optogenetic actuators. LITOS can uniformly stimulate all the cells in a well and allows for flexible temporal stimulation schemes. LITOS's affordability and ease of use aims at democratizing optogenetics in any laboratory.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Höhener, Thomas Christoph; Dessauges, Coralie; Hinderling, Lucien Simon; Gagliardi, Paolo Armando and Pertz, Olivier

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

02 Aug 2022 13:59

Last Modified:

02 Aug 2022 14:08

Publisher DOI:

10.1038/s41598-022-17312-x

PubMed ID:

35907941

BORIS DOI:

10.48350/171668

URI:

https://boris.unibe.ch/id/eprint/171668

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