Höhener, Thomas Christoph; Landolt, Alex Erich; Dessauges, Coralie; Hinderling, Lucien; Gagliardi, Paolo Armando; Pertz, Olivier (2022). LITOS: a versatile LED illumination tool for optogenetic stimulation. Scientific reports, 12(1), p. 13139. Springer Nature 10.1038/s41598-022-17312-x
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Optogenetics has become a key tool to manipulate biological processes with high spatio-temporal resolution. Recently, a number of commercial and open-source multi-well illumination devices have been developed to provide throughput in optogenetics experiments. However, available commercial devices remain expensive and lack flexibility, while open-source solutions require programming knowledge and/or include complex assembly processes. We present a LED Illumination Tool for Optogenetic Stimulation (LITOS) based on an assembled printed circuit board controlling a commercially available 32 × 64 LED matrix as illumination source. LITOS can be quickly assembled without any soldering, and includes an easy-to-use interface, accessible via a website hosted on the device itself. Complex light stimulation patterns can easily be programmed without coding expertise. LITOS can be used with different formats of multi-well plates, petri dishes, and flasks. We validated LITOS by measuring the activity of the MAPK/ERK signaling pathway in response to different dynamic light stimulation regimes using FGFR1 and Raf optogenetic actuators. LITOS can uniformly stimulate all the cells in a well and allows for flexible temporal stimulation schemes. LITOS's affordability and ease of use aims at democratizing optogenetics in any laboratory.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 09 Interdisciplinary Units > Microscopy Imaging Center (MIC) |
UniBE Contributor: |
Höhener, Thomas Christoph, Dessauges, Coralie, Hinderling, Lucien Simon, Gagliardi, Paolo Armando, Pertz, Olivier |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
2045-2322 |
Publisher: |
Springer Nature |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
02 Aug 2022 13:59 |
Last Modified: |
05 Dec 2022 16:22 |
Publisher DOI: |
10.1038/s41598-022-17312-x |
PubMed ID: |
35907941 |
BORIS DOI: |
10.48350/171668 |
URI: |
https://boris.unibe.ch/id/eprint/171668 |
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