Monocyte biology conserved across species: Functional insights from cattle.

Talker, Stephanie C; Barut, G Tuba; Lischer, Heidi E L; Rufener, Reto; von Münchow, Lilly; Bruggmann, Rémy; Summerfield, Artur (2022). Monocyte biology conserved across species: Functional insights from cattle. Frontiers in immunology, 13, p. 889175. Frontiers Research Foundation 10.3389/fimmu.2022.889175

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Similar to human monocytes, bovine monocytes can be split into CD14highCD16- classical, CD14highCD16high intermediate and CD14-/dimCD16high nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Talker, Stephanie; Barut, Güliz Tuba; Tschanz-Lischer, Heidi Erika Lisa; Rufener, Reto; Bruggmann, Rémy and Summerfield, Artur

Subjects:

600 Technology > 630 Agriculture

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Pubmed Import

Date Deposited:

16 Aug 2022 10:30

Last Modified:

05 Dec 2022 16:22

Publisher DOI:

10.3389/fimmu.2022.889175

PubMed ID:

35967310

Uncontrolled Keywords:

bovine monocyte subsets cattle classical monocytes immunometabolism intermediate monocytes nonclassical monocytes single-cell RNA sequencing (scRNA-seq) transcriptome

BORIS DOI:

10.48350/172043

URI:

https://boris.unibe.ch/id/eprint/172043

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