Upper keratinocytes of psoriatic skin lesions express high levels of NAP-1/IL-8 mRNA in situ.

Gillitzer, Reinhard; Berger, Rudolf; Mielke, Volker; Müller, Christoph; Wolff, Klaus; Stingl, Georg (1991). Upper keratinocytes of psoriatic skin lesions express high levels of NAP-1/IL-8 mRNA in situ. Journal of investigative dermatology, 97(1), pp. 73-79. Elsevier 10.1111/1523-1747.ep12478128

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In order to better understand the factors regulating disease promotion and activity in psoriasis (PS), we searched for the in situ expression of mRNA for various cytokines in long-standing PS skin lesions. Specific hybridization with a NAP-1/IL-8 anti-sense RNA probe was keratinocyte associated and yielded strong and specific signals exclusively in the upper layers of the lesional epidermis, but not in uninvolved skin from psoriatic patients or normal skin from non-psoriatics. Interestingly, NAP-1/IL-8 transcripts were focally clustered in a spotty pattern predominantly between the tips of elongated papillae, but were absent in the lower epidermal region and the dermal compartment. We consistently failed to detect appreciable numbers of TNF-alpha and/or IL-6 mRNA-containing cells in psoriatic lesions. These results support the notion that IL-8, rather than IL-6, is an important disease-promoting cytokine in PS. In view of the known in vitro and in vivo effects of IL-8, it is conceivable that this substance greatly contributes to the major pathologic changes seen in psoriatic skin, i.e., keratinocyte hyperproliferation and leucocyte infiltration. In this case, local pharmacologic down-regulation of NAP-1/IL-8 activity could be a promising therapeutic strategy in PS.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Müller, Christoph

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0022-202X

Publisher:

Elsevier

Language:

English

Submitter:

Christoph Müller

Date Deposited:

22 Sep 2022 14:23

Last Modified:

22 Sep 2022 14:30

Publisher DOI:

10.1111/1523-1747.ep12478128

PubMed ID:

1711550

BORIS DOI:

10.48350/172934

URI:

https://boris.unibe.ch/id/eprint/172934

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