CD5- CD8 alpha beta intestinal intraepithelial lymphocytes (IEL) are induced to express CD5 upon antigen-specific activation: CD5- and CD5+ CD8 alpha beta IEL do not represent separate T cell lineages.

Müller, Stefan; Jungo, Myriam; Aichele, Peter; Mueller, Christoph (1997). CD5- CD8 alpha beta intestinal intraepithelial lymphocytes (IEL) are induced to express CD5 upon antigen-specific activation: CD5- and CD5+ CD8 alpha beta IEL do not represent separate T cell lineages. European journal of immunology, 27(7), pp. 1756-1761. Wiley-VCH 10.1002/eji.1830270724

[img] Text
Eur_J_Immunol_-_2005_-_M_ller_-_CD5_CD8_intestinal_intraepithelial_lymphocytes_IEL_are_induced_to_express_CD5_upon.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (726kB) | Request a copy

We followed alpha beta T cell receptor (TCR) usage in subsets of gut intraepithelial lymphocytes (IEL) in major histocompatibility complex class I-restricted alpha beta TCR-transgenic (tg) mice. The proportion of tg alpha beta TCR+ CD8 alpha beta IEL is reduced compared with CD8+ splenocytes of the same animal, particularly under conventional conditions of maintenance. Further fractionation of CD8 alpha beta IEL according to the expression level of surface CD5 revealed that in conventionally housed animals tg TCR+ CD5- CD8 alpha beta IEL are as frequent as in specific pathogen-free (SPF) mice, whereas tg TCR+ CD5int or, even more pronounced, tg TCR+ CD5hi CD8 alpha beta IEL are greatly diminished when compared with mice kept under SPF conditions. Upon antigen-specific stimulation of CD5- CD8 alpha beta IEL in vitro, CD5 surface expression is up-regulated on a large fraction of cells within 48 h. Up-regulation of CD5 surface expression is further enhanced by the presence of the anti-alpha IEL monoclonal antibody 2E7. This clearly demonstrates that CD5-, and CD5+ CD8 alpha beta IEL cannot be considered as separate T cell lineages.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Müller, Christoph

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0014-2980

Publisher:

Wiley-VCH

Language:

English

Submitter:

Christoph Müller

Date Deposited:

23 Sep 2022 09:06

Last Modified:

23 Sep 2022 09:15

Publisher DOI:

10.1002/eji.1830270724

PubMed ID:

9247588

BORIS DOI:

10.48350/172962

URI:

https://boris.unibe.ch/id/eprint/172962

Actions (login required)

Edit item Edit item
Provide Feedback