The C-X-C chemokine ENA-78 is preferentially expressed in intestinal epithelium in inflammatory bowel disease.

Z'Graggen, Kaspar; Walz, Alfred; Mazzucchelli, Luca; Strieter, Robert M; Mueller, Christoph (1997). The C-X-C chemokine ENA-78 is preferentially expressed in intestinal epithelium in inflammatory bowel disease. Gastroenterology, 113(3), pp. 808-816. Elsevier 10.1016/s0016-5085(97)70175-6

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BACKGROUND & AIMS

Secretion of chemokines by epithelial cells may represent a crucial event in the pathogenesis of inflammatory bowel disease (IBD). Expression of the chemokine epithelial neutrophil-activating peptide 78 (ENA-78) was monitored in patients with IBD and normal controls.

METHODS

In situ hybridizations were performed on 41 tissue specimens from 15 patients with IBD and 10 controls to detect ENA-78 messenger RNA (mRNA). Immunofluorescence stainings were used to localize ENA-78 protein.

RESULTS

Intestinal epithelial cells expressing ENA-78 mRNA at detectable levels are found at comparable frequencies in patients with Crohn's disease and ulcerative colitis. Tissue specimens with mild to moderate histological signs of disease activity show slightly higher frequencies of ENA-78 mRNA-expressing epithelial cells than areas with signs of severe disease activity (P = 0.14). Immunofluorescence stainings showed presence of the ENA-78 protein in > 90% of preserved epithelial cells in IBD, in control tissues, ENA-78 mRNA was not detectable, and ENA-78 protein was detectable in 0%-30% of epithelial cells.

CONCLUSIONS

The observations are in agreement with a role of the C-X-C chemokine ENA-78 in the pathogenesis of IBD.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Müller, Christoph

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0016-5085

Publisher:

Elsevier

Language:

English

Submitter:

Christoph Müller

Date Deposited:

22 Sep 2022 14:12

Last Modified:

22 Sep 2022 14:21

Publisher DOI:

10.1016/s0016-5085(97)70175-6

PubMed ID:

9287972

BORIS DOI:

10.48350/172963

URI:

https://boris.unibe.ch/id/eprint/172963

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