Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection.

Démoulins, Thomas; Brügger, Melanie; Zumkehr, Beatrice; Oliveira Esteves, Blandina I; Ruggli, Nicolas; Alves, Marco (2022). Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection. STAR protocols, 3(4), p. 101688. Elsevier 10.1016/j.xpro.2022.101688

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Here, we present a protocol to analyze the T cell profiles of the neonatal ovine lung during respiratory syncytial virus (RSV) infection. The protocol delivers standardized multiparameter flow cytometry (FCM) analysis of CD4+, CD8+, regulatory, and γδ T cells isolated from lung, lymph nodes, and bronchoalveolar lavages (BALs). We detail the preparation of RSV and transtracheal inoculation of newborn lambs. We then describe tissue isolation and preparation of cell suspensions, followed by FCM acquisition to identify different T cell subsets. For complete details on the use and execution of this protocol, please refer to Démoulins et al. (2021).

Item Type:

Journal Article (Further Contribution)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Démoulins, Thomas Paul Rémi, Brügger, Melanie, Zumkehr, Béatrice, Oliveira Esteves Criblez, Blandina Isabel, Ruggli, Nicolas, Alves, Marco

Subjects:

600 Technology > 630 Agriculture

ISSN:

2666-1667

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

20 Sep 2022 14:02

Last Modified:

21 Dec 2022 00:13

Publisher DOI:

10.1016/j.xpro.2022.101688

PubMed ID:

36116074

Uncontrolled Keywords:

Flow cytometry/Mass cytometry Health sciences Immunology Microbiology Model organisms

BORIS DOI:

10.48350/173068

URI:

https://boris.unibe.ch/id/eprint/173068

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