Reynoso-Moreno, Ines; Rau, Mark; Chicca, Andrea; Nicolussi, Simon; Gertsch, Jürg (2023). Assay of Endocannabinoid Uptake. Methods in molecular biology, 2576, pp. 329-348. Springer 10.1007/978-1-0716-2728-0_28
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Endocannabinoids at nanomolar physiological concentrations cross cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics and endocannabinoid trafficking employing radioligands and mass spectrometry. Here, we describe radiosubstrate-based assays using arachidonoyl[1-3H]ethanolamine and 2-arachidonoyl[1,2,3-3H]glycerol to measure cellular endocannabinoid uptake in a three-phase assay with human U937 cells. Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)-based lipidomics was used to interrogate the roles of serum and albumin for endocannabinoid trafficking in U937 cells.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Reynoso, Ines del Carmen, Rau, Mark, Chicca, Andrea, Nicolussi, Simon, Gertsch, Jürg |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1940-6029 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
26 Sep 2022 13:48 |
Last Modified: |
05 Dec 2022 16:25 |
Publisher DOI: |
10.1007/978-1-0716-2728-0_28 |
PubMed ID: |
36152200 |
Uncontrolled Keywords: |
2-Arachidonoylglycerol Anandamide Endocannabinoid uptake LC–ESI–MS/MS |
BORIS DOI: |
10.48350/173258 |
URI: |
https://boris.unibe.ch/id/eprint/173258 |
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