Placement of Biological Membrane Patches in a Nanofluidic Gap With Control Over Position and Orientation

Ruggeri, Francesca; Schwemmer, Christian; Stauffer, Mirko; Nicollier, Philippe; Figueiredo da Silva, Jacqueline; Bosshart, Patrick D; Kochems, Kirstin; Fotiadis, Dimitrios; Knoll, Armin; Wolf, Heiko (2022). Placement of Biological Membrane Patches in a Nanofluidic Gap With Control Over Position and Orientation. Advanced materials interfaces, 9(23), p. 2200941. Wiley 10.1002/admi.202200941

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Purple membranes from the archaeon Halobacterium salinarum consist of 2D crystals of the light-driven proton pump bacteriorhodopsin, which convert photons into a proton gradient across the cell membrane. This functional feature and the structural rigidity make them appealing candidates for integration into biomimetic devices. To this end, and in order to carry out their function, purple membranes must be positioned in the correct orientation at
the position of interest. Precise placement and control over the orientation of nanoscale objects still constitutes a formidable challenge. Here, it is shown that isolated purple membrane patches can be transported and positioned at predefined locations in nanofluidic confinement, with control over their orientation at the target sites. The transport is achieved through a rocking Brownian motor scheme, while the controlled deposition of the membranes
is realized by engineering the surface potential of a fluid-filled nanofluidic slit. This controlled manipulation of purple membrane patches outlines a new pathway toward the integration of biological or other delicate supramolecular structures into top–down-fabricated patterns, for the assembly of nanoscale hybrid devices that serve as a light-driven source of (chemical) energy.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Stauffer, Mirko, Bosshart, Patrick, Fotiadis, Dimitrios José

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2196-7350

Publisher:

Wiley

Language:

English

Submitter:

Barbara Franziska Järmann-Bangerter

Date Deposited:

17 Nov 2022 15:23

Last Modified:

05 Dec 2022 16:28

Publisher DOI:

10.1002/admi.202200941

BORIS DOI:

10.48350/174844

URI:

https://boris.unibe.ch/id/eprint/174844

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