A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle

Dejea, Hector; Schlepütz, Christian M.; Méndez-Carmona, Natalia; Arnold, Maria; Garcia-Canadilla, Patricia; Longnus, Sarah L.; Stampanoni, Marco; Bijnens, Bart; Bonnin, Anne (2022). A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle. Frontiers in cardiovascular medicine, 9 Frontiers 10.3389/fcvm.2022.1023483

[img]
Preview
Text
fcvm-09-1023483.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (4MB) | Preview

ntroduction: Cardiac architecture has been extensively investigated ex vivo using a broad spectrum of imaging techniques. Nevertheless, the heart is a dynamic system and the structural mechanisms governing the cardiac cycle can only be unveiled when investigating it as such.

Methods: This work presents the customization of an isolated, perfused heart system compatible with synchrotron-based X-ray phase contrast imaging (X-PCI).

Results: Thanks to the capabilities of the developed setup, it was possible to visualize a beating isolated, perfused rat heart for the very first time in 4D at an unprecedented 2.75 μm pixel size (10.6 μm spatial resolution), and 1 ms temporal resolution.

Discussion: The customized setup allows high-spatial resolution studies of heart architecture along the cardiac cycle and has thus the potential to serve as a tool for the characterization of the structural dynamics of the heart, including the effects of drugs and other substances able to modify the cardiac cycle.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Heart Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Herz- und Gefässchirurgie

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Méndez Carmona, Natalia, Arnold, Maria Regula, Henning Longnus, Sarah

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2297-055X

Publisher:

Frontiers

Language:

English

Submitter:

Paul Libera

Date Deposited:

05 Jan 2023 08:23

Last Modified:

27 Feb 2024 14:28

Publisher DOI:

10.3389/fcvm.2022.1023483

BORIS DOI:

10.48350/176462

URI:

https://boris.unibe.ch/id/eprint/176462

Actions (login required)

Edit item Edit item
Provide Feedback