Mc Kinnon, Brett D; Lukowski, Samuel W; Mortlock, Sally; Crawford, Joanna; Atluri, Sharat; Subramaniam, Sugarniya; Johnston, Rebecca L; Nirgianakis, Konstantinos; Tanaka, Keisuke; Amoako, Akwasi; Mueller, Michael D; Montgomery, Grant W (2022). Altered differentiation of endometrial mesenchymal stromal fibroblasts is associated with endometriosis susceptibility. Communications biology, 5(1), p. 600. Springer Nature 10.1038/s42003-022-03541-3
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Cellular development is tightly regulated as mature cells with aberrant functions may initiate pathogenic processes. The endometrium is a highly regenerative tissue, shedding and regenerating each month. Endometrial stromal fibroblasts are regenerated each cycle from mesenchymal stem cells and play a pivotal role in endometriosis, a disease characterised by endometrial cells that grow outside the uterus. Why the cells of some women are more capable of developing into endometriosis lesions is not clear. Using isolated, purified and cultured endometrial cells of mesenchymal origin from 19 women with (n = 10) and without (n = 9) endometriosis we analysed the transcriptome of 33,758 individual cells and compared these to clinical characteristics and in vitro growth profiles. We show purified mesenchymal cell cultures include a mix of mesenchymal stem cells and two endometrial stromal fibroblast subtypes with distinct transcriptomic signatures indicative of varied progression through the differentiation processes. The fibroblast subgroup characterised by incomplete differentiation was predominantly (81%) derived from women with endometriosis and exhibited an altered in vitro growth profile. These results uncover an inherent difference in endometrial cells of women with endometriosis and highlight the relevance of cellular differentiation and its potential to contribute to disease susceptibility.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
Mc Kinnon, Brett, Nirgianakis, Konstantinos, Mueller, Michael |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2399-3642 |
Publisher: |
Springer Nature |
Language: |
English |
Submitter: |
Monika Zehr |
Date Deposited: |
11 Jan 2023 17:08 |
Last Modified: |
11 Jan 2023 23:23 |
Publisher DOI: |
10.1038/s42003-022-03541-3 |
PubMed ID: |
35725766 |
BORIS DOI: |
10.48350/176826 |
URI: |
https://boris.unibe.ch/id/eprint/176826 |
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