Kara, Nilufer; Ayoub, Nooraldeen; Ilgu, Huseyin; Fotiadis, Dimitrios; Ilgu, Muslum (2023). Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications. Molecules, 28(9) MDPI 10.3390/molecules28093728
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Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These "aptasensors" can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.
Item Type: |
Journal Article (Review Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Ayoub, Nooraldeen Fathi Nooraldeen, Ilgü, Hüseyin, Fotiadis, Dimitrios José |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1420-3049 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
15 May 2023 13:56 |
Last Modified: |
07 Aug 2024 15:44 |
Publisher DOI: |
10.3390/molecules28093728 |
PubMed ID: |
37175137 |
Uncontrolled Keywords: |
SELEX aptamers aptasensor biosensor diagnostics membrane proteins |
BORIS DOI: |
10.48350/182532 |
URI: |
https://boris.unibe.ch/id/eprint/182532 |
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