Jahnke, Laura; Zandi, Souska; Elhelbawi, Ahmed; Conedera, Federica Maria; Enzmann, Volker (2023). Characterization of Macroglia Response during Tissue Repair in a Laser-Induced Model of Retinal Degeneration. International journal of molecular sciences, 24(11), p. 9172. MDPI 10.3390/ijms24119172
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Reactive gliosis is a hallmark of chronic degenerative diseases of the retina. As gliosis involves macroglia, we investigated their gliotic response to determine the role of S100β and intermediate filaments (IFs) GFAP, vimentin, and nestin during tissue repair in a laser-induced model of retinal degeneration. We validated the results with human retinal donor samples. Ex-periments were performed in zebrafish and mice using an argon laser (532 nm) to induce focal lesions in the outer retina. At different time points following injury induction, the kinetics of retinal degeneration and regeneration were assessed using hematoxylin and eosin staining (H&E). Immunofluorescence was performed to evaluate Müller cell (GS) and astrocyte (GFAP) injury response and to distinguish between both cell types. Additionally, staining was per-formed in human retinal sections containing drusen. Focal laser treatment elevated the expres-sion of gliotic markers in the area of the damage, which was associated with increased expres-sion of S100β, GFAP, vimentin, and nestin in mice and humans. In zebrafish, we detected S100β at the first time point, but not GFAP or nestin. Double-positive cells with the selected glia markers were detected in all models. However, in zebrafish, no double-positive GFAP/GS cells were found on days 10 and 17, nor were S100β/GS double-positive cells found on day 12. Macro-glia cells showed a different pattern in the expression of IFs in degenerative and regenerative models. In particular, S100β may prove to be a target for suppressing chronic gliosis in retinal degeneration.
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