Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue.

Díaz-Alejo, Jesús Frutos; April-Monn, Simon; Cihova, Marina; Buocikova, Verona; Villalón López, Jorge; Urbanova, Maria; Lechuga, Carmen G; Tomas, Miroslav; Dubovan, Peter; Sánchez, Bárbara Luna; Páez, Sonia Camaño; Sanjuanbenito, Alfonso; Lobo, Eduardo; Romio de la Heras, Estefanía; Guerra, Carmen; de la Pinta, Carolina; Barreto Melian, Emma; Rodríguez Garrote, Mercedes; Carrato, Alfredo; Ruiz-Cañas, Laura; ... (2023). Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue. Journal of visualized experiments(195) MYJoVE Corporation 10.3791/65229

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Tumor organoids are three-dimensional (3D) ex vivo tumor models that recapitulate the biological key features of the original primary tumor tissues. Patient-derived tumor organoids have been used in translational cancer research and can be applied to assess treatment sensitivity and resistance, cell-cell interactions, and tumor cell interactions with the tumor microenvironment. Tumor organoids are complex culture systems that require advanced cell culture techniques and culture media with specific growth factor cocktails and a biological basement membrane that mimics the extracellular environment. The ability to establish primary tumor cultures highly depends on the tissue of origin, the cellularity, and the clinical features of the tumor, such as the tumor grade. Furthermore, tissue sample collection, material quality and quantity, as well as correct biobanking and storage are crucial elements of this procedure. The technical capabilities of the laboratory are also crucial factors to consider. Here, we report a validated SOP/protocol that is technically and economically feasible for the culture of ex vivo tumor organoids from fresh tissue samples of pancreatic adenocarcinoma origin, either from fresh primary resected patient donor tissue or patient-derived xenografts (PDX). The technique described herein can be performed in laboratories with basic tissue culture and mouse facilities and is tailored for wide application in the translational oncology field.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Pathology
UniBE Contributor:	April-Monn, Simon
Subjects:	500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health
ISSN:	1940-087X
Publisher:	MYJoVE Corporation
Language:	English
Submitter:	Pubmed Import
Date Deposited:	13 Jun 2023 10:52
Last Modified:	13 Jun 2023 10:52
Publisher DOI:	10.3791/65229
PubMed ID:	37306424
Additional Information:	Jesús Frutos Díaz-Alejo and Simon April-Monn contributed equally.
URI:	https://boris.unibe.ch/id/eprint/183360