Parallels between oncogene-driven cardiac hyperplasia and heart regeneration in zebrafish.

Pfefferli, Catherine; Bonvin, Marylène; Grepper, Dogan; Robatel, Steve; König, Désirée; Lischer, Heidi E L; Bruggmann, Rémy; Jaźwińska, Anna (2023). Parallels between oncogene-driven cardiac hyperplasia and heart regeneration in zebrafish. Development, 150(12) The Company of Biologists 10.1242/dev.201412

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The human heart is poorly regenerative and cardiac tumors are extremely rare. Whether the adult zebrafish myocardium is responsive to oncogene overexpression and how this condition affects its intrinsic regenerative capacity remains unknown. Here, we have established a strategy of inducible and reversible expression of HRASG12V in zebrafish cardiomyocytes. This approach stimulated a hyperplastic cardiac enlargement within 16 days. The phenotype was suppressed by rapamycin-mediated inhibition of TOR signaling. As TOR signaling is also required for heart restoration after cryoinjury, we compared transcriptomes of hyperplastic and regenerating ventricles. Both conditions were associated with upregulation of cardiomyocyte dedifferentiation and proliferation factors, as well as with similar microenvironmental responses, such as deposition of nonfibrillar Collagen XII and recruitment of immune cells. Among the differentially expressed genes, many proteasome and cell-cycle regulators were upregulated only in oncogene-expressing hearts. Preconditioning of the heart with short-term oncogene expression accelerated cardiac regeneration after cryoinjury, revealing a beneficial synergism between both programs. Identification of the molecular bases underlying the interplay between detrimental hyperplasia and advantageous regeneration provides new insights into cardiac plasticity in adult zebrafish.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Biology > Bioinformatics and Computational Biology

UniBE Contributor:

Tschanz-Lischer, Heidi Erika Lisa, Bruggmann, Rémy




The Company of Biologists




Pubmed Import

Date Deposited:

15 Jun 2023 08:07

Last Modified:

15 Jun 2023 08:09

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PubMed ID:


Uncontrolled Keywords:

Cardiomegaly Gal4-ERT2 RAS oncogene Regeneration TOR Zebrafish heart




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