Labode, Jonas; Haberthür, David; Hlushchuk, Ruslan; Regin, Yannick; Gie, Andre George; Salaets, Thomas; Toelen, Jaan; Mühlfeld, Christian (2023). Location-specific pathology analysis of the monopodial pulmonary vasculature in a rabbit model of bronchopulmonary dysplasia-A pilot study. Physiological reports, 11(12), e15747. The American Physiological Society 10.14814/phy2.15747
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Physiological_Reports_-_2023_-_Labode_-_Location_specific_pathology_analysis_of_the_monopodial_pulmonary_vasculature_in_a.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (5MB) | Preview |
The mammalian pulmonary vasculature consists of functionally and morphologically heterogeneous compartments. When comparing sets of lungs, for example, in disease models or therapeutic interventions, local changes may be masked by the overall heterogeneity of the organ structure. Therefore, alterations taking place only in a sub-compartment may not be detectable by global analysis. In the monopodial lung, the characterization of distinct vessel groups is difficult, due to the asymmetrical branching pattern. In this pilot study, a previously established method to classify segments of the monopodial pulmonary arterial tree into homogeneous groups was employed. To test its suitability for experimental settings, the method was applied to a hyperoxia (HYX, ≥95% oxygen) rabbit model of bronchopulmonary dysplasia and a normoxic control group (NOX, 21% oxygen). The method allowed the identification of morphological differences between the HYX and the NOX groups. Globally visible differences in lumen diameter were pinpointed to specific lung regions. Furthermore, local changes of wall dimension and cell layers in single compartments, that would not have been identifiable in an unfocused analysis of the whole dataset, were found. In conclusion, the described method achieves a higher precision in morphological studies of lung disease models, compared to a common, global analysis approach.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy |
UniBE Contributor: |
Haberthür, David, Hlushchuk, Ruslan |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2051-817X |
Publisher: |
The American Physiological Society |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Jun 2023 11:50 |
Last Modified: |
16 Nov 2023 15:36 |
Publisher DOI: |
10.14814/phy2.15747 |
PubMed ID: |
37358021 |
Uncontrolled Keywords: |
branching analysis cluster analysis light microscopy microcomputed tomography monopodial lung pulmonary vasculature |
BORIS DOI: |
10.48350/184163 |
URI: |
https://boris.unibe.ch/id/eprint/184163 |
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