de Melo, Guilherme Dias; Perraud, Victoire; Alvarez, Flavio; Vieites-Prado, Alba; Kim, Seonhee; Kergoat, Lauriane; Coleon, Anthony; Trüeb, Bettina Salome; Tichit, Magali; Piazza, Aurèle; Thierry, Agnès; Hardy, David; Wolff, Nicolas; Munier, Sandie; Koszul, Romain; Simon-Lorière, Etienne; Thiel, Volker; Lecuit, Marc; Lledo, Pierre-Marie; Renier, Nicolas; ... (2023). Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants. Nature communications, 14(1), p. 4485. Nature Publishing Group 10.1038/s41467-023-40228-7
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Anosmia was identified as a hallmark of COVID-19 early in the pandemic, however, with the emergence of variants of concern, the clinical profile induced by SARS-CoV-2 infection has changed, with anosmia being less frequent. Here, we assessed the clinical, olfactory and neuroinflammatory conditions of golden hamsters infected with the original Wuhan SARS-CoV-2 strain, its isogenic ORF7-deletion mutant and three variants: Gamma, Delta, and Omicron/BA.1. We show that infected animals develop a variant-dependent clinical disease including anosmia, and that the ORF7 of SARS-CoV-2 contributes to the induction of olfactory dysfunction. Conversely, all SARS-CoV-2 variants are neuroinvasive, regardless of the clinical presentation they induce. Taken together, this confirms that neuroinvasion and anosmia are independent phenomena upon SARS-CoV-2 infection. Using newly generated nanoluciferase-expressing SARS-CoV-2, we validate the olfactory pathway as a major entry point into the brain in vivo and demonstrate in vitro that SARS-CoV-2 travels retrogradely and anterogradely along axons in microfluidic neuron-epithelial networks.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Other Institutions > Centers Vetsuisse Faculty > Multidisciplinary Center for Infectious Diseases (MCID) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Trüeb, Bettina Salome, Thiel, Volker Earl |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
2041-1723 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Jul 2023 13:32 |
Last Modified: |
10 Dec 2023 02:24 |
Publisher DOI: |
10.1038/s41467-023-40228-7 |
Related URLs: |
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PubMed ID: |
37495586 |
BORIS DOI: |
10.48350/185085 |
URI: |
https://boris.unibe.ch/id/eprint/185085 |
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