Czyrnik, Elena D; Wiesehöfer, Marc; Dankert, Jaroslaw T; Wach, Sven; Wagner, Mathias; Spahn, Martin; Kruithof de Julio, Marianna; Wennemuth, Gunther (2023). Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells. Frontiers in oncology, 13(1212585), p. 1212585. Frontiers Research Foundation 10.3389/fonc.2023.1212585
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INTRODUCTION
Cell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly interact to maintain homeostasis or tissue integrity. This study focuses on an indirect communication via soluble factors.
METHODS
To investigate the cell-cell interaction via soluble factors, the prostate carcinoma cell line LNCaP and the stromal primary cells p21 were co-cultured without direct contact and RNA was isolated at defined time points. Differences in gene expression were finally analyzed by RNA sequencing.
RESULTS
RNA sequencing revealed a time-depending differential expression profile. Selected factors were subsequently characterized at molecular level and analyzed in human prostate tissue of different developmental stages as well as pathology. GALNT14 was one of the highest induced co-culture-specific genes in LNCaP cells. Detection in healthy tissue and BPH revealed an age-dependent decrease in GALNT14 expression. Moreover, in prostate carcinoma, GALNT14 expression heavily varied independent of the Gleason score.
CONCLUSION
Overall, this work provides a basis for further studies related to paracrine stromal-epithelial interaction in prostate carcinoma and highlights the importance of GALNT14.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Urologie 04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology |
UniBE Contributor: |
Kruithof-de Julio, Marianna |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2234-943X |
Publisher: |
Frontiers Research Foundation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
07 Sep 2023 12:01 |
Last Modified: |
29 Oct 2023 02:22 |
Publisher DOI: |
10.3389/fonc.2023.1212585 |
PubMed ID: |
37671061 |
Uncontrolled Keywords: |
GALNT14 co-culture paracrine interaction prostate carcinoma prostate tissue stromal-epithelial interaction |
BORIS DOI: |
10.48350/186102 |
URI: |
https://boris.unibe.ch/id/eprint/186102 |
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