Milusev, Anastasia; Ren, Jianfang; Despont, Alain; Shaw, Jane; Längin, Matthias; Bender, Martin; Abicht, Jan-Michael; Mokelke, Maren; Radan, Julia; Neumann, Elisabeth; Kemter, Elisabeth; Klymiuk, Nikolai; Ayares, David; Wolf, Eckhard; Reichart, Bruno; Sorvillo, Nicoletta; Rieben, Robert (2023). Glycocalyx dynamics and the inflammatory response of genetically modified porcine endothelial cells. Xenotransplantation, 30(5), e12820. Wiley 10.1111/xen.12820
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Xenotransplantation_-_2023_-_Milusev.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (6MB) | Preview |
Xenotransplantation is a promising approach to reduce organ shortage, while genetic modification of donor pigs has significantly decreased the immunogenic burden of xenotransplants, organ rejection is still a hurdle. Genetically modified pig organs are used in xenotransplantation research, and the first clinical pig-to-human heart transplantation was performed in 2022. However, the impact of genetic modification has not been investigated on a cellular level yet. Endothelial cells (EC) and their sugar-rich surface known as the glycocalyx are the first barrier encountering the recipient's immune system, making them a target for rejection. We have previously shown that wild type venous but not arterial EC were protected against heparan sulfate (HS) shedding after activation with human serum or human tumor necrosis factor alpha (TNF
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