Pharmacological inhibition of bromodomain and extra-terminal proteins induces an NRF-2-mediated antiviral state that is subverted by SARS-CoV-2 infection.

Mhlekude, Baxolele; Postmus, Dylan; Stenzel, Saskia; Weiner, January; Jansen, Jenny; Zapatero-Belinchón, Francisco J; Olmer, Ruth; Richter, Anja; Heinze, Julian; Heinemann, Nicolas; Mühlemann, Barbara; Schroeder, Simon; Jones, Terry C; Müller, Marcel A; Drosten, Christian; Pich, Andreas; Thiel, Volker; Martin, Ulrich; Niemeyer, Daniela; Gerold, Gisa; ... (2023). Pharmacological inhibition of bromodomain and extra-terminal proteins induces an NRF-2-mediated antiviral state that is subverted by SARS-CoV-2 infection. PLoS pathogens, 19(9), e1011657. Public Library of Science 10.1371/journal.ppat.1011657

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Inhibitors of bromodomain and extra-terminal proteins (iBETs), including JQ-1, have been suggested as potential prophylactics against SARS-CoV-2 infection. However, molecular mechanisms underlying JQ-1-mediated antiviral activity and its susceptibility to viral subversion remain incompletely understood. Pretreatment of cells with iBETs inhibited infection by SARS-CoV-2 variants and SARS-CoV, but not MERS-CoV. The antiviral activity manifested itself by reduced reporter expression of recombinant viruses, and reduced viral RNA quantities and infectious titers in the culture supernatant. While we confirmed JQ-1-mediated downregulation of expression of angiotensin-converting enzyme 2 (ACE2) and interferon-stimulated genes (ISGs), multi-omics analysis addressing the chromatin accessibility, transcriptome and proteome uncovered induction of an antiviral nuclear factor erythroid 2-related factor 2 (NRF-2)-mediated cytoprotective response as an additional mechanism through which JQ-1 inhibits SARS-CoV-2 replication. Pharmacological inhibition of NRF-2, and knockdown of NRF-2 and its target genes reduced JQ-1-mediated inhibition of SARS-CoV-2 replication. Serial passaging of SARS-CoV-2 in the presence of JQ-1 resulted in predominance of ORF6-deficient variant, which exhibited resistance to JQ-1 and increased sensitivity to exogenously administered type I interferon (IFN-I), suggesting a minimised need for SARS-CoV-2 ORF6-mediated repression of IFN signalling in the presence of JQ-1. Importantly, JQ-1 exhibited a transient antiviral activity when administered prophylactically in human airway bronchial epithelial cells (hBAECs), which was gradually subverted by SARS-CoV-2, and no antiviral activity when administered therapeutically following an established infection. We propose that JQ-1 exerts pleiotropic effects that collectively induce an antiviral state in the host, which is ultimately nullified by SARS-CoV-2 infection, raising questions about the clinical suitability of the iBETs in the context of COVID-19.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Thiel, Volker Earl

Subjects:

600 Technology > 630 Agriculture

ISSN:

1553-7366

Publisher:

Public Library of Science

Language:

English

Submitter:

Pubmed Import

Date Deposited:

26 Sep 2023 11:20

Last Modified:

08 Nov 2023 00:15

Publisher DOI:

10.1371/journal.ppat.1011657

PubMed ID:

37747932

BORIS DOI:

10.48350/186588

URI:

https://boris.unibe.ch/id/eprint/186588

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