Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1.

Stojanovski, Klement; Gheorghe, Ioana; Lenart, Peter; Lanjuin, Anne; Mair, William B; Towbin, Benjamin D (2023). Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1. Nature Communications, 14(1), p. 7564. Springer Nature 10.1038/s41467-023-43230-1

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Even slight imbalance between the growth rate of different organs can accumulate to a large deviation from their appropriate size during development. Here, we use live imaging of the pharynx of C. elegans to ask if and how organ size scaling nevertheless remains uniform among individuals. Growth trajectories of hundreds of individuals reveal that pharynxes grow by a near constant volume per larval stage that is independent of their initial size, such that undersized pharynxes catch-up in size during development. Tissue-specific depletion of RAGA-1, an activator of mTOR and growth, shows that maintaining correct pharynx-to-body size proportions involves a bi-directional coupling between pharynx size and body growth. In simulations, this coupling cannot be explained by limitation of food uptake alone, and genetic experiments reveal an involvement of the mechanotransducing transcriptional co-regulator yap-1. Our data suggests that mechanotransduction coordinates pharynx growth with other tissues, ensuring body plan uniformity among individuals.

Item Type:

Journal Article (Original Article)

Division/Institute:

09 Interdisciplinary Units > Microscopy Imaging Center (MIC)
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Stojanovski, Klement, Gheorghe, Ioana, Lenart, Peter, Towbin, Benjamin Daniel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2041-1723

Publisher:

Springer Nature

Language:

English

Submitter:

Pubmed Import

Date Deposited:

22 Nov 2023 11:31

Last Modified:

19 Sep 2024 09:17

Publisher DOI:

10.1038/s41467-023-43230-1

PubMed ID:

37985670

BORIS DOI:

10.48350/189243

URI:

https://boris.unibe.ch/id/eprint/189243

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