Beyeler, Raphael; Jordan, Melanie; Dorner, Lilian; He, Buyuan; Cyrklaff, Marek; Roques, Magali; Stanway, Rebecca; Frischknecht, Friedrich; Heussler, Volker (2024). Putative prefoldin complex subunit 5 of Plasmodium berghei is crucial for microtubule formation and parasite development in the mosquito. Molecular microbiology, 121(3), pp. 481-496. Blackwell Science 10.1111/mmi.15196
Full text not available from this repository. (Request a copy)Plasmodium sporozoite development in and egress from oocysts in the Anopheles mosquito remains largely enigmatic. In a previously performed high-throughput knockout screen, the putative subunit 5 of the prefoldin complex (PbPCS5, PBANKA_0920100) was identified as essential for parasite development during mosquito and liver stage development. Here we generated and analyzed a PbPCS5 knockout parasite line during its development in the mosquito. Interestingly, PbPCS5 deletion does not significantly affect oocyst formation but leads to a growth defect resulting in aberrantly shaped sporozoites. Sporozoites produced in the absence of PbPCS5 were thinner, markedly elongated, and did, in most cases, not contain a nucleus. Sporozoites contained fewer subpellicular microtubules, which reached deep into the sporoblast during sporogony where they contacted and indented nuclei. These aberrantly shaped sporozoites did not reach the salivary glands, and we, therefore, conclude that PbPCS5 is essential for sporogony and the life cycle progression of the parasite during its mosquito stage.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Beyeler, Raphael Nicola, Roques, Magali Hélène Yvonne, Heussler, Volker |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0950-382X |
Publisher: |
Blackwell Science |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
27 Nov 2023 15:45 |
Last Modified: |
12 Mar 2024 00:13 |
Publisher DOI: |
10.1111/mmi.15196 |
PubMed ID: |
38009402 |
Uncontrolled Keywords: |
Plasmodium microtubule mosquito stage oocyst prefoldin complex sporozoite |
URI: |
https://boris.unibe.ch/id/eprint/189463 |
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