Ghenu, Ana-Hermina; Amado, André; Gordo, Isabel; Bank, Claudia (2023). Epistasis decreases with increasing antibiotic pressure but not temperature. Philosophical transactions of the Royal Society. Series B - biological sciences, 378(1877), pp. 1-13. Royal Society of London 10.1098/rstb.2022.0058
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ghenu-et-al-2023-epistasis-decreases-with-increasing-antibiotic-pressure-but-not-temperature.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (1MB) | Preview |
Predicting mutational effects is essential for the control of antibiotic resistance (ABR). Predictions are difficult when there are strong genotype-byenvironment (G× E), gene-by-gene (G ×G or epistatic) or gene-by-geneby-environment (G ×G× E) interactions. We quantified G×G× E effects in Escherichia coli across environmental gradients. We created intergenic fitness landscapes using gene knock-outs and single-nucleotide ABR mutations previously identified to vary in the extent of G× E effects in our environments of interest. Then,we measured competitive fitness acrossacomplete combinatorial
set of temperature and antibiotic dosage gradients. In this way, we assessed the predictability of 15 fitness landscapes across 12 different but related environments. We found G×G interactions and rugged fitness landscapes in the absence of antibiotic, but as antibiotic concentration increased, the fitness effects of ABR genotypes quickly overshadowed those of gene knock-outs, and the
landscapes became smoother. Our work reiterates that some single mutants, like those conferring resistance or susceptibility to antibiotics, have consistent effects across genetic backgrounds in stressful environments. Thus, although epistasis may reduce the predictability of evolution in benign environments, evolution may be more predictable in adverse environments. This article is part of the theme issue ‘Interdisciplinary approaches to predicting evolutionary biology’.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) 08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Theoretical Ecology and Evolution |
UniBE Contributor: |
Ghenu, Ana-Hermina, da Conceição Amado, André, Bank, Claudia |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
0962-8436 |
Publisher: |
Royal Society of London |
Language: |
English |
Submitter: |
Susanne Holenstein |
Date Deposited: |
09 Feb 2024 16:39 |
Last Modified: |
09 Feb 2024 16:39 |
Publisher DOI: |
10.1098/rstb.2022.0058 |
PubMed ID: |
37004727 |
Uncontrolled Keywords: |
environmental interactions, cryptic epistasis, RNA polymerase B (rpoB), genetic interactions, rifampicin |
BORIS DOI: |
10.48350/192740 |
URI: |
https://boris.unibe.ch/id/eprint/192740 |
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