Harder, Daniel; Ritzmann, Noah; Ucurum Fotiadis, Zöhre; Müller, Daniel J; Fotiadis, Dimitrios (2024). Light Color-Controlled pH-Adjustment of Aqueous Solutions Using Engineered Proteoliposomes. Advanced science, 11(15), e2307524. Wiley 10.1002/advs.202307524
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Controlling the pH at the microliter scale can be useful for applications in research, medicine, and industry, and therefore represents a valuable application for synthetic biology and microfluidics. The presented vesicular system translates light of different colors into specific pH changes in the surrounding solution. It works with the two light-driven proton pumps bacteriorhodopsin and blue light-absorbing proteorhodopsin Med12, that are oriented in opposite directions in the lipid membrane. A computer-controlled measuring device implements a feedback loop for automatic adjustment and maintenance of a selected pH value. A pH range spanning more than two units can be established, providing fine temporal and pH resolution. As an application example, a pH-sensitive enzyme reaction is presented where the light color controls the reaction progress. In summary, light color-controlled pH-adjustment using engineered proteoliposomes opens new possibilities to control processes at the microliter scale in different contexts, such as in synthetic biology applications.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Harder, Daniel, Ucurum Fotiadis, Zöhre, Fotiadis, Dimitrios José |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2198-3844 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
12 Feb 2024 12:25 |
Last Modified: |
18 Apr 2024 00:14 |
Publisher DOI: |
10.1002/advs.202307524 |
PubMed ID: |
38342618 |
Uncontrolled Keywords: |
bacteriorhodopsin light-driven proton pump proteoliposome proteorhodopsin synthetic biology |
BORIS DOI: |
10.48350/192812 |
URI: |
https://boris.unibe.ch/id/eprint/192812 |
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