Bernstein, Mariana; Rudzinski, Marcelo; Schneider, Vanesa; Messina, Matías; Gos, María Laura; Helman, Elisa; Dellarupe, Andrea; Unzaga, Juan Manuel; Venturini, María Cecilia; Moré, Gastón; Pardini, Lais (2024). Genetic characterization of Toxoplasma gondii from human and chicken isolates from Argentina. Parasitology research, 123(2) Springer-Verlag 10.1007/s00436-024-08142-z
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This study aimed to determine the nPCR-RFLP genotypes of newly obtained T. gondii isolates from human congenital
toxoplasmosis cases in Argentina and to determine their allelic profiles for virulence genes ROP18/ROP5. In addition,
the ROP18/ROP5 profiles were also determined for previously characterized T. gondii samples. Isolation from congenital
toxoplasmosis cases was carried out in mouse bioassay from two placentas (P1 and P2). Genotyping for the new human
isolates was performed by nPCR-RFLP using 10 markers. The samples analyzed for ROP18/ROP5 included the two newly
obtained isolates (from the congenital toxoplasmosis cases) and nine previously genotyped T. gondii DNA samples from
humans and chickens. The results for P1 and P2 named as TgHm18-02Arg and TgHm19-01Arg showed ToxoDB genotypes
#14 (non-archetypal) and #2 (clonal type III), respectively. Non-archetypal #14 has been isolated from human cases before
in Argentina. However, this is the first report of T. gondii clonal type III in a human case in the country. The ROP18/ROP5
combination was detected in nine samples: 3/3 (n = 1), 4/3 (n = 4), 4/4 (n = 3), and 3–4/4 (n = 1). Notably, the 4/4 profile was
identified for the first time and exclusively in T. gondii samples from Misiones province (which borders southern Brazil).
Further studies are required to corroborate the regionalization of the ROP18/ROP5 profiles in Argentina.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology |
UniBE Contributor: |
Moré, Gastón Andrés |
Subjects: |
500 Science > 590 Animals (Zoology) 600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture |
ISSN: |
0932-0113 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Pamela Schumacher |
Date Deposited: |
20 Mar 2024 14:16 |
Last Modified: |
20 Mar 2024 14:16 |
Publisher DOI: |
10.1007/s00436-024-08142-z |
PubMed ID: |
38332310 |
BORIS DOI: |
10.48350/194311 |
URI: |
https://boris.unibe.ch/id/eprint/194311 |
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