Physiological-dependent alterations on transcriptomic and proteomic patterns of the single and combined temperature and salinity-exposed hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂

Bo, Jun; Zheng, Ronghui; Jiang, Yulu; Chen, Jincan; Fang, Chao; Bailey, Christyn; Zhang, Yusheng; Lee, Jae-Seong; Segner, Helmut (2023). Physiological-dependent alterations on transcriptomic and proteomic patterns of the single and combined temperature and salinity-exposed hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂. Aquaculture, 575 Elsevier 10.1016/j.aquaculture.2023.739746

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Global warming and the changes in environmental conditions such as temperature and salinity may affect the
health of marine species. However, the underlying mechanisms remain poorly characterized. The objectives of
the present study were to examine the liver transcriptome and proteome of the marine species, hybrid grouper
(E. fuscoguttatus ♀ × E. lanceolatus ♂), to assess which physiological pathways are modulated by exposure to
climate change-related stressors, how the responses vary with exposure duration, and whether they represent
adaptive or maladaptive changes. To answer these questions, the hybrid grouper was subjected to single stressors
(elevated temperatures, reduced salinity) or stressor combinations for 3 or 14 days. As endpoints, we examined
changes in body and organ growth, liver histopathology, lipid accumulation, and alterations of the liver transcriptome
and proteome. The results demonstrated that the elevated temperature resulted in reduced body
weight and a reduced liver-somatic index after a 14-day exposure. The spleen-somatic index was significantly
decreased compared to controls after 3-day treatment. At the transcriptomic level, the pathways with the greatest
numbers of differentially expressed genes at day 3 of exposure were immune-related in all treatment groups.
After 14 days of exposure, the transcriptomic and proteomic analyses showed that metabolic and protective
processes became activated. The increased lipid droplet accumulation in the hepatocytes is corroborated by the
transcriptomic/proteomic findings which show alterations in the pathways of lipid metabolism and fatty acid
oxidation. While these changes may represent adaptive responses, the increase of hepatic lipid accumulation
despite an overall loss of liver and body weight may represent a maladaptive process. Overall, the physiological
responses of the hybrid grouper showed a clear time dependency, and they were partly stressor-specific, with the
low salinity treatment separating from the treatments with elevated temperature. When both stressors were
combined, the temperature effect dominated the salinity effect.

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