Bindschedler, Annina; Schmuckli-Maurer, Jacqueline; Buchser, Sophie; Fischer, Tara D; Wacker, Rahel; Davalan, Tim; Brunner, Jessica; Heussler, Volker T (2024). LC3B labeling of the parasitophorous vacuole membrane of Plasmodium berghei liver stage parasites depends on the V-ATPase and ATG16L1. Molecular microbiology, 121(6), pp. 1095-1111. Wiley 10.1111/mmi.15259
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Molecular_Microbiology_-_2024_-_Bindschedler_-_LC3B_labeling_of_the_parasitophorous_vacuole_membrane_of_Plasmodium_berghei.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (22MB) | Preview |
The protozoan parasite Plasmodium, the causative agent of malaria, undergoes an obligatory stage of intra-hepatic development before initiating a blood-stage infection. Productive invasion of hepatocytes involves the formation of a parasitophorous vacuole (PV) generated by the invagination of the host cell plasma membrane. Surrounded by the PV membrane (PVM), the parasite undergoes extensive replication. During intracellular development in the hepatocyte, the parasites provoke the Plasmodium-associated autophagy-related (PAAR) response. This is characterized by a long-lasting association of the autophagy marker protein, and ATG8 family member, LC3B with the PVM. LC3B localization at the PVM does not follow the canonical autophagy pathway since upstream events specific to canonical autophagy are dispensable. Here, we describe that LC3B localization at the PVM of Plasmodium parasites requires the V-ATPase and its interaction with ATG16L1. The WD40 domain of ATG16L1 is crucial for its recruitment to the PVM. Thus, we provide new mechanistic insight into the previously described PAAR response targeting Plasmodium liver stage parasites.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Bindschedler, Annina Flavia, Schmuckli, Jacqueline, Wacker, Rahel Corina, Davalan, Tim Nico, Heussler, Volker |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1365-2958 |
Publisher: |
Wiley |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
08 Apr 2024 12:18 |
Last Modified: |
14 Jun 2024 00:13 |
Publisher DOI: |
10.1111/mmi.15259 |
PubMed ID: |
38574236 |
Uncontrolled Keywords: |
Plasmodium CASM autophagy host‐parasite interaction liver stage |
BORIS DOI: |
10.48350/195676 |
URI: |
https://boris.unibe.ch/id/eprint/195676 |
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