Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe.

Ivanova, Mirena; Ovsepian, Armen; Leekitcharoenphon, Pimlapas; Seyfarth, Anne Mette; Mordhorst, Hanne; Otani, Saria; Koeberl-Jelovcan, Sandra; Milanov, Mihail; Kompes, Gordan; Liapi, Maria; Černý, Tomáš; Vester, Camilla Thougaard; Perrin-Guyomard, Agnès; Hammerl, Jens A; Grobbel, Mirjam; Valkanou, Eleni; Jánosi, Szilárd; Slowey, Rosemarie; Alba, Patricia; Carfora, Virginia; ... (2024). Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe. (In Press). The journal of antimicrobial chemotherapy Oxford University Press 10.1093/jac/dkae161

Preview

Text dkae161.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (849kB) | Preview

OBJECTIVES

To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within the EU harmonized antimicrobial resistance (AMR) surveillance programme in 2014-18 and the Danish AMR surveillance programme in 2016-19.

METHODS

WGS data of 1007 E. coli [165 azithromycin resistant (MIC > 16 mg/L)] and 269 Salmonella [29 azithromycin resistant (MIC > 16 mg/L)] were screened for acquired macrolide resistance genes and mutations in rplDV, 23S rRNA and acrB genes using ResFinder v4.0, AMRFinder Plus and custom scripts. Genotype-phenotype concordance was determined for all isolates. Transferability of mef(C)-mph(G)-carrying plasmids was assessed by conjugation experiments.

RESULTS

mph(A), mph(B), mef(B), erm(B) and mef(C)-mph(G) were detected in E. coli and Salmonella, whereas erm(C), erm(42), ere(A) and mph(E)-msr(E) were detected in E. coli only. The presence of macrolide resistance genes, alone or in combination, was concordant with the azithromycin-resistant phenotype in 69% of isolates. Distinct mph(A) operon structures were observed in azithromycin-susceptible (n = 50) and -resistant (n = 136) isolates. mef(C)-mph(G) were detected in porcine and bovine E. coli and in porcine Salmonella enterica serovar Derby and Salmonella enterica 1,4, [5],12:i:-, flanked downstream by ISCR2 or TnAs1 and associated with IncIγ and IncFII plasmids.

CONCLUSIONS

Diverse azithromycin resistance genes were detected in E. coli and Salmonella from food-producing animals and meat in Europe. Azithromycin resistance genes mef(C)-mph(G) and erm(42) appear to be emerging primarily in porcine E. coli isolates. The identification of distinct mph(A) operon structures in susceptible and resistant isolates increases the predictive power of WGS-based methods for in silico detection of azithromycin resistance in Enterobacterales.

Interest & Impact

Downloads

0 since deposited on 23 May 2024
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item