Ivanova, Mirena; Ovsepian, Armen; Leekitcharoenphon, Pimlapas; Seyfarth, Anne Mette; Mordhorst, Hanne; Otani, Saria; Koeberl-Jelovcan, Sandra; Milanov, Mihail; Kompes, Gordan; Liapi, Maria; Černý, Tomáš; Vester, Camilla Thougaard; Perrin-Guyomard, Agnès; Hammerl, Jens A; Grobbel, Mirjam; Valkanou, Eleni; Jánosi, Szilárd; Slowey, Rosemarie; Alba, Patricia; Carfora, Virginia; ... (2024). Azithromycin resistance in Escherichia coli and Salmonella from food-producing animals and meat in Europe. The journal of antimicrobial chemotherapy, 79(7), pp. 1657-1667. Oxford University Press 10.1093/jac/dkae161
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OBJECTIVES
To characterize the genetic basis of azithromycin resistance in Escherichia coli and Salmonella collected within the EU harmonized antimicrobial resistance (AMR) surveillance programme in 2014-18 and the Danish AMR surveillance programme in 2016-19.
METHODS
WGS data of 1007 E. coli [165 azithromycin resistant (MIC > 16 mg/L)] and 269 Salmonella [29 azithromycin resistant (MIC > 16 mg/L)] were screened for acquired macrolide resistance genes and mutations in rplDV, 23S rRNA and acrB genes using ResFinder v4.0, AMRFinder Plus and custom scripts. Genotype-phenotype concordance was determined for all isolates. Transferability of mef(C)-mph(G)-carrying plasmids was assessed by conjugation experiments.
RESULTS
mph(A), mph(B), mef(B), erm(B) and mef(C)-mph(G) were detected in E. coli and Salmonella, whereas erm(C), erm(42), ere(A) and mph(E)-msr(E) were detected in E. coli only. The presence of macrolide resistance genes, alone or in combination, was concordant with the azithromycin-resistant phenotype in 69% of isolates. Distinct mph(A) operon structures were observed in azithromycin-susceptible (n = 50) and -resistant (n = 136) isolates. mef(C)-mph(G) were detected in porcine and bovine E. coli and in porcine Salmonella enterica serovar Derby and Salmonella enterica 1,4, [5],12:i:-, flanked downstream by ISCR2 or TnAs1 and associated with IncIγ and IncFII plasmids.
CONCLUSIONS
Diverse azithromycin resistance genes were detected in E. coli and Salmonella from food-producing animals and meat in Europe. Azithromycin resistance genes mef(C)-mph(G) and erm(42) appear to be emerging primarily in porcine E. coli isolates. The identification of distinct mph(A) operon structures in susceptible and resistant isolates increases the predictive power of WGS-based methods for in silico detection of azithromycin resistance in Enterobacterales.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Overesch, Gudrun |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1460-2091 |
Publisher: |
Oxford University Press |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
23 May 2024 08:21 |
Last Modified: |
02 Jul 2024 00:14 |
Publisher DOI: |
10.1093/jac/dkae161 |
PubMed ID: |
38775752 |
BORIS DOI: |
10.48350/197033 |
URI: |
https://boris.unibe.ch/id/eprint/197033 |
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