New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome.

Démoulins, Thomas; Techakriengkrai, Navapon; Ebensen, Thomas; Schulze, Kai; Liniger, Matthias; Gerber, Markus; Nedumpun, Teerawut; McCullough, Kenneth C; Guzmán, Carlos A; Suradhat, Sanipa; Ruggli, Nicolas (2024). New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome. Methods in molecular biology, 2786, pp. 89-133. Springer 10.1007/978-1-0716-3770-8_4

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While mRNA vaccines have shown their worth, they have the same failing as inactivated vaccines, namely they have limited half-life, are non-replicating, and therefore limited to the size of the vaccine payload for the amount of material translated. New advances averting these problems are combining replicon RNA (RepRNA) technology with nanotechnology. RepRNA are large self-replicating RNA molecules (typically 12-15 kb) derived from viral genomes defective in at least one essential structural protein gene. They provide sustained antigen production, effectively increasing vaccine antigen payloads over time, without the risk of producing infectious progeny. The major limitations with RepRNA are RNase-sensitivity and inefficient uptake by dendritic cells (DCs), which need to be overcome for efficacious RNA-based vaccine design. We employed biodegradable delivery vehicles to protect the RepRNA and promote DC delivery. Condensing RepRNA with polyethylenimine (PEI) and encapsulating RepRNA into novel Coatsome-replicon vehicles are two approaches that have proven effective for delivery to DCs and induction of immune responses in vivo.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Démoulins, Thomas Paul Rémi, Liniger, Matthias, Gerber, Markus Daniel, McCullough, Kenneth, Ruggli, Nicolas

Subjects:

600 Technology > 630 Agriculture

ISSN:

1940-6029

Publisher:

Springer

Language:

English

Submitter:

Pubmed Import

Date Deposited:

31 May 2024 15:36

Last Modified:

03 Jun 2024 13:13

Publisher DOI:

10.1007/978-1-0716-3770-8_4

PubMed ID:

38814391

Uncontrolled Keywords:

Coatsome-replicon vehicles Polyplexes Porcine reproductive and respiratory syndrome virus RepRNA Replicon RNA SARS-CoV-2 Self-replicating vaccine Severe acute respiratory syndrome coronavirus 2 Universal influenza vaccine c-di-AMP

BORIS DOI:

10.48350/197406

URI:

https://boris.unibe.ch/id/eprint/197406

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