Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes

De Pascale, M Clara; Bassi, Anna Maria; Patrone, Vania; Villacorta, Luis; Azzi, Angelo; Zingg, Jean-Marc (2006). Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes. Archives of biochemistry and biophysics, 447(2), pp. 97-106. San Diego, Calif.: Elsevier 10.1016/j.abb.2006.02.002

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In skin, vitamin E acts as the predominant lipophilic antioxidant with a protective function against irradiation and oxidative stress. In addition to that, vitamin E can also modulate signal transduction and gene expression. To study whether the four natural tocopherol analogues (alpha-, beta-, gamma-, delta-tocopherol) can influence transcriptional activity by modulating the activity of nuclear receptors, a human keratinocytes cell line (NCTC 2544) was transfected with plasmids containing the luciferase reporter gene under control by direct repeat elements (DR1-DR4), representing binding sites for four different classes of nuclear receptors. In this model, the tocopherols positively modulated only the reporter construct containing a consensus element for peroxisome proliferator-activated receptors (PPARs). The induction was strongest with gamma-tocopherol and was most likely the direct consequence of stimulation of PPARgamma protein expression in keratinocytes. Vitamin E treatment also led to increased expression of a known PPARgamma target gene involved in terminal keratinocytes differentiation, the transglutaminase-1.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Azzi, Angelo and Zingg, Jean-Marc

ISSN:

0003-9861

ISBN:

16530159

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:49

Last Modified:

06 Dec 2013 13:43

Publisher DOI:

10.1016/j.abb.2006.02.002

PubMed ID:

16530159

Web of Science ID:

000236526400001

URI:

https://boris.unibe.ch/id/eprint/20379 (FactScience: 3667)

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