Granulocyte colony-stimulating factor supports liver regeneration in a small-for-size liver remnant mouse model

Inderbitzin, Daniel; Beldi, Guido; Sidler, Daniel; Studer, Peter; Keogh, Adrian; Bisch-Knaden, Sonja; Weimann, Rosy; Kappeler, Andreas; Gloor, Beat; Candinas, Daniel (2007). Granulocyte colony-stimulating factor supports liver regeneration in a small-for-size liver remnant mouse model. Journal of gastrointestinal surgery, 11(3), pp. 280-5. New York, N.Y.: Springer-Verlag 10.1007/s11605-007-0096-0

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Experimental partial hepatectomy of more than 80% of the liver weight bears an increased mortality in rodents, due to impaired hepatic regeneration in small-for-size liver remnants. Granulocyte colony-stimulating factor (G-CSF) promotes progenitor cell expansion and mobilization and also has immunomodulatory properties. The aim of this study was to determine the effect of systemically administered G-CSF on liver regeneration and animal survival in a small-for-size liver remnant mouse model. Mice were preconditioned daily for 5 days with subcutaneous injections of 5 microg G-CSF or aqua ad injectabile. Subsequently, 83% partial hepatectomy was performed by resecting the median, the left, the caudate, and the right inferior hepatic lobes in all animals. Daily sham or G-CSF injection was continued. Survival was significantly better in G-CSF-treated animals (P < 0.0001). At 36 and 48 h after microsurgical hepatic resection, markers of hepatic proliferation (Ki67, BrdU) were elevated in G-CSF-treated mice compared to sham injected control animals (P < 0.0001) and dry liver weight was increased (P < 0.05). G-CSF conditioning might prove to be useful in patients with small-for-size liver remnants after extended hepatic resections due to primary or secondary liver tumors or in the setting of split liver transplantation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Inderbitzin, Daniel; Beldi, Guido; Studer, Peter; Weimann, Rosemarie; Kappeler, Andreas; Gloor, Beat and Candinas, Daniel

ISSN:

1091-255X

ISBN:

17458598

Publisher:

Springer-Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:53

Last Modified:

26 Jun 2018 13:49

Publisher DOI:

10.1007/s11605-007-0096-0

PubMed ID:

17458598

Web of Science ID:

000245949900007

BORIS DOI:

10.7892/boris.22640

URI:

https://boris.unibe.ch/id/eprint/22640 (FactScience: 35696)

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