Polymorphism and chromosomal location of the MC4R (melanocortin-4 receptor) gene in the dog and red fox

Skorczyk, Anna; Stachowiak, Monika; Szczerbal, Izabela; Klukowska-Roetzler, Jolanta; Schelling, Claude; Dolf, Gaudenz; Switonski, Marek (2007). Polymorphism and chromosomal location of the MC4R (melanocortin-4 receptor) gene in the dog and red fox. Gene, 392(1-2), pp. 247-52. Amsterdam: Elsevier 10.1016/j.gene.2006.12.027

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The melanocortin-4 receptor (MC4R) is expressed in the hypothalamus and regulates energy intake and body weight. In silico screening of the canine chromosome 1 sequence and a comparison with the porcine MC4R sequence by BLAST were performed. The nucleotide sequence of the whole coding region and 3'- and 5'-flanking regions of the dog (1214 bp) and red fox (1177 bp) MC4R gene was established and high conservation of the nucleotide sequences was revealed (99%). Five sets of PCR primers were designed and a search for polymorphism was performed by the SSCP technique in a group of 31 dogs representing nineteen breeds and 35 farm red foxes. Sequencing of DNA fragments, representing the identified SSCP patterns, revealed three single nucleotide polymorphisms (including a missense one) in dogs and four silent SNPs in red foxes. An average SNP frequency was approx. 1/400 bp in the dog and 1/300 bp in the red fox. We mapped the MC4R gene by FISH to the canine chromosome 1 (CFA1q1.1) and to the red fox chromosome 5 (VVU5p1.2).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics

UniBE Contributor:

Klukowska, Jolanta and Dolf, Gaudenz

ISSN:

0378-1119

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:53

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1016/j.gene.2006.12.027

PubMed ID:

17306938

Web of Science ID:

000246078300028

URI:

https://boris.unibe.ch/id/eprint/22716 (FactScience: 36213)

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