Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray

de Souza Luna, Luciano Kleber; Heiser, Volker; Regamey, Nicolas; Panning, Marcus; Drexler, Jan Felix; Mulangu, Sabue; Poon, Leo; Baumgarte, Sigrid; Haijema, Bert Jan; Kaiser, Laurent; Drosten, Christian (2007). Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray. Journal of clinical microbiology, 45(3), pp. 1049-52. Washington, D.C.: American Society for Microbiology 10.1128/JCM.02426-06

Full text not available from this repository. (Request a copy)

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Regamey, Nicolas

ISSN:

0095-1137

ISBN:

17229859

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:55

Last Modified:

04 May 2014 23:16

Publisher DOI:

10.1128/JCM.02426-06

PubMed ID:

17229859

Web of Science ID:

000245071500058

URI:

https://boris.unibe.ch/id/eprint/23438 (FactScience: 41823)

Actions (login required)

Edit item Edit item
Provide Feedback