Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray

de Souza Luna, Luciano Kleber; Heiser, Volker; Regamey, Nicolas; Panning, Marcus; Drexler, Jan Felix; Mulangu, Sabue; Poon, Leo; Baumgarte, Sigrid; Haijema, Bert Jan; Kaiser, Laurent; Drosten, Christian (2007). Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray. Journal of clinical microbiology, 45(3), pp. 1049-52. Washington, D.C.: American Society for Microbiology 10.1128/JCM.02426-06

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A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
UniBE Contributor:	Regamey, Nicolas
ISSN:	0095-1137
ISBN:	17229859
Publisher:	American Society for Microbiology
Language:	English
Submitter:	Anette van Dorland
Date Deposited:	04 Oct 2013 14:55
Last Modified:	05 Dec 2022 14:17
Publisher DOI:	10.1128/JCM.02426-06
PubMed ID:	17229859
Web of Science ID:	000245071500058
URI:	https://boris.unibe.ch/id/eprint/23438 (FactScience: 41823)