A homologue of aliB is found in the capsule region of nonencapsulated Streptococcus pneumoniae

Hathaway, LJ; Stutzmann Meier, P; Bättig, P; Aebi, S; Mühlemann, K (2004). A homologue of aliB is found in the capsule region of nonencapsulated Streptococcus pneumoniae. Journal of bacteriology, 186(12), pp. 3721-9. Washington, D.C.: American Society for Microbiology 10.1128/JB.186.12.3721-3729.2004

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The epidemiology, phylogeny, and biology of nonencapsulated Streptococcus pneumoniae are largely unknown. Increased colonization capacity and transformability are, however, intriguing features of these pneumococci and play an important role. Twenty-seven nonencapsulated pneumococci were identified in a nationwide collection of 1,980 nasopharyngeal samples and 215 blood samples obtained between 1998 and 2002. On the basis of multilocus sequence typing and capsule region analysis we divided the nonencapsulated pneumococci into two groups. Group I was closely related to encapsulated strains. Group II had a clonal population structure, including two geographically widespread clones able to cause epidemic conjunctivitis and invasive diseases. Group II strains also carried a 1,959-bp homologue of aliB (aliB-like ORF 2) in the capsule region, which was highly homologous to a sequence in the capsule region of Streptococcus mitis. In addition, strains of the two major clones in group II had an additional sequence, aliB-like ORF 1 (1,968 to 2,004 bp), upstream of aliB-like ORF 2. Expression of aliB-like ORF 1 was detected by reverse transcription-PCR, and the corresponding RNA was visualized by Northern blotting. A gene fragment homologous to capN of serotypes 33 and 37 suggests that group II strains were derived from encapsulated pneumococci some time ago. Therefore, loss of capsule expression in vivo was found to be associated with the importation of one or two aliB homologues in some nonencapsulated pneumococci.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Stutzmann, Patricia

ISSN:

0021-9193

ISBN:

15175285

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:56

Last Modified:

04 May 2014 23:16

Publisher DOI:

10.1128/JB.186.12.3721-3729.2004

PubMed ID:

15175285

Web of Science ID:

000221869100008

URI:

https://boris.unibe.ch/id/eprint/23944 (FactScience: 45315)

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