Severe structural damage of the seemingly non-diseased infrarenal aortic aneurysm neck

Diehm, Nicolas; Di Santo, Stefano; Schaffner, Thomas; Schmidli, Juerg; Völzmann, Jan; Jüni, Peter; Baumgartner, Iris; Kalka, Christoph (2008). Severe structural damage of the seemingly non-diseased infrarenal aortic aneurysm neck. Journal of vascular surgery, 48(2), pp. 425-34. Amsterdam: Elsevier 10.1016/j.jvs.2008.03.001

[img] Text
Diehm JVascSurg 2008.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

OBJECTIVE: The success of open and endovascular repair of abdominal aortic aneurysms (AAA) is hampered by postoperative dilatation of the anatomical neck of the AAA, which is used for graft attachment. The purpose of this study was to determine whether the macroscopically non-diseased infrarenal aortic neck of AAA is histologically and biochemically altered at the time of operative repair. METHODS: We harvested full-thickness aortic wall samples as longitudinal stripes spanning from AAA neck to aneurysmal sac in 22 consecutive patients undergoing open surgical AAA repair. Control tissue was obtained from five organ donors and five deceased subjects undergoing autopsy without evidence of aneurysmal disease. We assessed aortic media thickness, number of intact elastic lamellar units, media destruction, and neovascularization grade and performed immunohistochemistry for matrix metalloproteinase (MMP)-9 and phosphorylated c-Jun N-terminal kinase (p-JNK). MMP-9 and p-JNK protein expressions were quantified using Western Blots. RESULTS: The median thickness of the aortic media was 1150 mum in control tissue (range, 1000-1300), 510 mum in aortic necks (250-900), and 200 mum in aortic sacs (50-500, P from nonparametric test for trend <.001). The number of intact elastic lamellar units was 33 in controls (range, 33-55), 12 in aortic necks (0-31) and three in aortic sacs (0-10, P < .001). The expression of MMP-9 and p-JNK as assessed by Western Blots (P = .007 and .061, respectively) and zymography (P for trend <.001) were up regulated in both the AAA neck and sac compared with controls. Except for p-JNK expression, differences between tissues were similar after the adjustment for age, gender, and type of sampling. CONCLUSION: The seemingly non-diseased infrarenal AAA neck in patients with AAA undergoing surgical repair shows histological signs of destruction and upregulation of potential drug targets.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiovascular Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine

UniBE Contributor:

Diehm, Nicolas Alexander; Di Santo, Stefano; Schaffner, Thomas; Schmidli, Jürg; Jüni, Peter; Baumgartner, Iris and Kalka, Christoph

ISSN:

0741-5214

ISBN:

18502079

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

09 Jun 2015 16:04

Publisher DOI:

10.1016/j.jvs.2008.03.001

PubMed ID:

18502079

Web of Science ID:

000258035800029

BORIS DOI:

10.7892/boris.27258

URI:

https://boris.unibe.ch/id/eprint/27258 (FactScience: 105436)

Actions (login required)

Edit item Edit item
Provide Feedback