The transcription factor IFN regulatory factor-4 controls experimental colitis in mice via T cell-derived IL-6

Mudter, Jonas; Amoussina, Lioubov; Schenk, Mirjam; Yu, Jingling; Brüstle, Anne; Weigmann, Benno; Atreya, Raja; Wirtz, Stefan; Becker, Christoph; Hoffman, Arthur; Atreya, Imke; Biesterfeld, Stefan; Galle, Peter R; Lehr, Hans A; Rose-John, Stefan; Mueller, Christoph; Lohoff, Michael; Neurath, Markus F (2008). The transcription factor IFN regulatory factor-4 controls experimental colitis in mice via T cell-derived IL-6. Journal of clinical investigation, 118(7), pp. 2415-26. Ann Arbor, Mich.: American Society for Clinical Investigation 10.1172/JCI33227

[img]
Preview
Text
render.pdf - Published Version
Available under License Publisher holds Copyright.

Download (1MB) | Preview

The proinflammatory cytokine IL-6 seems to have an important role in the intestinal inflammation that characterizes inflammatory bowel diseases (IBDs) such as Crohn disease and ulcerative colitis. However, little is known about the molecular mechanisms regulating IL-6 production in IBD. Here, we assessed the role of the transcriptional regulator IFN regulatory factor-4 (IRF4) in this process. Patients with either Crohn disease or ulcerative colitis exhibited increased IRF4 expression in lamina propria CD3+ T cells as compared with control patients. Consistent with IRF4 having a regulatory function in T cells, in a mouse model of IBD whereby colitis is induced in RAG-deficient mice by transplantation with CD4+CD45RB(hi) T cells, adoptive transfer of wild-type but not IRF4-deficient T cells resulted in severe colitis. Furthermore, IRF4-deficient mice were protected from T cell-dependent chronic intestinal inflammation in trinitrobenzene sulfonic acid- and oxazolone-induced colitis. In addition, IRF4-deficient mice with induced colitis had reduced mucosal IL-6 production, and IRF4 was required for IL-6 production by mucosal CD90+ T cells, which it protected from apoptosis. Finally, the protective effect of IRF4 deficiency could be abrogated by systemic administration of either recombinant IL-6 or a combination of soluble IL-6 receptor (sIL-6R) plus IL-6 (hyper-IL-6). Taken together, our data identify IRF4 as a key regulator of mucosal IL-6 production in T cell-dependent experimental colitis and suggest that IRF4 might provide a therapeutic target for IBDs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Immunopathology
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Schenk, Mirjam and Müller, Christoph

ISSN:

0021-9738

ISBN:

18535667

Publisher:

American Society for Clinical Investigation

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:03

Last Modified:

22 Jul 2015 09:57

Publisher DOI:

10.1172/JCI33227

PubMed ID:

18535667

Web of Science ID:

000257657400010

BORIS DOI:

10.7892/boris.27340

URI:

https://boris.unibe.ch/id/eprint/27340 (FactScience: 106198)

Actions (login required)

Edit item Edit item
Provide Feedback