Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis

Ruhwald, M; Bodmer, T; Maier, C; Jepsen, M; Haaland, M B; Eugen-Olsen, J; Ravn, P (2008). Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis. European respiratory journal, 32(6), pp. 1607-15. Sheffield, UK: European Respiratory Society 10.1183/09031936.00055508

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The aim of the present study was to evaluate the potential of diagnostic tests based on interferon-gamma inducible protein (IP)-10 and monocyte chemotactic protein (MCP)-2, and compare the performance with the QuantiFERON TB Gold In-Tube (QFT-IT; Cellestis, Carnagie, Australia) test. IP-10 and MCP-2 were determined in supernatants from whole blood stimulated with Mycobacterium tuberculosis-specific antigens. Samples were obtained from 80 patients with culture- and/or PCR-proven tuberculosis (TB), and 124 unexposed healthy controls: 86 high school students and 38 high school staff. IP-10 and MCP-2 test cut-offs were established based on receiver operating characteristic curve analysis. TB patients produced significantly higher levels (median) of IP-10 (2158 pg x mL(-1)) and MCP-2 (379 pg x mL(-1)) compared with interferon (IFN)-gamma (215 pg x mL(-1)). The QFT-IT, IP-10 and MCP-2 tests detected 81, 83 and 71% of the TB patients; 0, 3 and 0% of the high school students and 0, 16 and 3% of the staff, respectively. Agreement between tests was high (>89%). By combining IP-10 and IFN-gamma tests, the detection rate increased among TB patients to 90% without a significant increase in positive responders among the students. In conclusion, interferon-gamma inducible protein-10 and monocyte chemotactic protein-2 responses to Mycobacterium tuberculosis-specific antigens could be used to diagnose infection. Combining interferon-gamma inducible protein-10 and interferon-gamma may be a simple approach to increase the detection rate of the Mycobacterium tuberculosis-specific in vitro tests.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Virology and Immunology
UniBE Contributor:	Bodmer, Thomas, Maier, Claudia
ISSN:	0903-1936
ISBN:	18684849
Publisher:	European Respiratory Society
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:06
Last Modified:	05 Dec 2022 14:20
Publisher DOI:	10.1183/09031936.00055508
PubMed ID:	18684849
Web of Science ID:	000261725200027
URI:	https://boris.unibe.ch/id/eprint/28674 (FactScience: 125126)