The feasibility of non-viral gene transfer to the diaphragm in vivo

Beshay, Morris; Gazdhar, Amiq; Gugger, Mathias; Reymond, Marc; Schmid, Ralph A (2009). The feasibility of non-viral gene transfer to the diaphragm in vivo. Development, growth & differentiation, 51(6), pp. 547-553. Richmond (Aus.): Blackwell Publishing Asia 10.1111/j.1440-169X.2009.01117.x

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Gene transfer using electroporation is an essential method for the study of developmental biology, especially to understand the internal control of degeneration and apoptosis of the muscle cells that occurs earlier and quicker than the usual degeneration process occurring by aging. Such experimental studies may have a role in developing new strategies for treating patients suffering from inherited primary myopathies such as Duchenne muscular dystrophy (DMD). The present study was designed to evaluate the feasibility of electroporation mediated transfer of reporter genes to the diaphragm in vivo. This is the first report of gene transfer of naked plasmid DNA into the diaphragm muscle in vivo using electroporation. Our results showed that in vivo gene transfer of naked plasmid DNA into the diaphragm muscle using electroporation is feasible.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute of Pathology 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
UniBE Contributor:	Gugger, Mathias, Schmid, Ralph
ISSN:	0012-1592
ISBN:	19594785
Publisher:	Blackwell Publishing Asia
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:08
Last Modified:	05 Dec 2022 14:21
Publisher DOI:	10.1111/j.1440-169X.2009.01117.x
PubMed ID:	19594785
Web of Science ID:	000268458500003
URI:	https://boris.unibe.ch/id/eprint/29989 (FactScience: 165612)