Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx

Brugger, Silvio D.; Hathaway, Lucy J; Mühlemann, Kathrin (2009). Detection of Streptococcus pneumoniae strain cocolonization in the nasopharynx. Journal of clinical microbiology, 47(6), pp. 1750-6. Washington, D.C.: American Society for Microbiology 10.1128/JCM.01877-08

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Colonization with more than one distinct strain of the same species, also termed cocolonization, is a prerequisite for horizontal gene transfer between pneumococcal strains that may lead to change of the capsular serotype. Capsule switch has become an important issue since the introduction of conjugated pneumococcal polysaccharide vaccines. There is, however, a lack of techniques to detect multiple colonization by S. pneumoniae strains directly in nasopharyngeal samples. Two hundred eighty-seven nasopharyngeal swabs collected during the prevaccine era within a nationwide surveillance program were analyzed by a novel technique for the detection of cocolonization, based on PCR amplification of a noncoding region adjacent to the pneumolysin gene (plyNCR) and restriction fragment length polymorphism (RFLP) analysis. The numbers of strains and their relative abundance in cocolonized samples were determined by terminal RFLP. The pneumococcal carriage rate found by PCR was 51.6%, compared to 40.0% found by culture. Cocolonization was present in 9.5% (10/105) of samples, most (9/10) of which contained two strains in a ratio of between 1:1 and 17:1. Five of the 10 cocolonized samples showed combinations of vaccine types only (n = 2) or combinations of nonvaccine types only (n = 3). Carriers of multiple pneumococcal strains had received recent antibiotic treatment more often than those colonized with a single strain (33% versus 9%, P = 0.025). This new technique allows for the rapid and economical study of pneumococcal cocolonization in nasopharyngeal swabs. It will be valuable for the surveillance of S. pneumoniae epidemiology under vaccine selection pressure.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of General Internal Medicine (DAIM) > Clinic of General Internal Medicine > Centre of Competence for General Internal Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Brugger, Silvio and Mühlemann, Kathrin

ISSN:

0095-1137

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:14

Last Modified:

24 Nov 2014 08:42

Publisher DOI:

10.1128/JCM.01877-08

PubMed ID:

19386843

Web of Science ID:

000266454400023

URI:

https://boris.unibe.ch/id/eprint/32435 (FactScience: 197641)

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